Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: Usefulness of Detecting Brain-Derived Neurotrophic Factor in Intervertebral Disc Degeneration of the Lumbosacral Spine
doi: 10.12659/MSM.938663
Figure Lengend Snippet: Expression of pro-BDNF in IVD obtained from the study and control groups, and annulus fibrosus, and nucleus pulposus from the control group obtained via the western blot technique. P – stage of radiological advancement of changes according to the Pfirrmann scale.
Article Snippet: Next, we added a primary mouse monoclonal IgG1 κ pro-BDNF antibody anti-BDNF antibody (Santa Cruz Biotech, Dallas, TX, USA; molecular weight 32 kDa; catalog number sc-65514) and incubated the whole thing overnight at 4°C with gentle shaking.
Techniques: Expressing, Control, Western Blot